Application

Immunoblotting (1:500)r>Immunofluorescence (1:1000, indirect)r>Immunoprecipitation (see comments)r>

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 mouse myeloma cells. Recognizes the ~180 kDa clathrin heavy chain protein.

This Anti-Clathrin Heavy Chain Mouse mAb (X22) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Clathrin Heavy Chain.

Recognizes the ~180 kDa clathrin heavy chain protein.

Immunogen

Human

clathrin heavy chain purified from human brain

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Immunoprecipitation of triskelions allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. Antibody should be titrated for optimal results in individual systems.

Bauerfeind, R., et al. 1996. J. Neurocytol.25, 701.Zhang, J.Z., et al. 1994. Cell78, 751.Herskovits, J.S., et al. 1993. J. Cell Biol.122, 565.Van Der Bliek, A.M., et al. 1993. J. Cell Biol.122, 553.Pearse, B.M. and Robinson, M.S. 1990. Ann. Rev. Cell Biol.6, 151.Brodsky, F.M. 1985. J. Cell Biol.101, 2047.

Packaging

100 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)